Fig. 11.     ChAT activity in forebrain regions 14 days after unilateral 6-OHDA injections (10 µg of 6-OHDA dissolved in 4 µl saline containing 1 mg/ml ascorbic acid) into the ascending catecholaminergic fiber bundle at the meso-diencephalic border. Values are expressed as percentage of the corresponding sham injections (vehicle only) where shaded columns are ipsilateral side and open columns are contralateral side. Maximal in vitro activity of ChAT was measured radiochemically using [³H]acetyl]-CoA (Luine and McEwen, l983). Protein was measured according to Bradford et al. (l976). ChAT activities reflect nmoles ACh produced/mg protein/hr and are mean + S.E.M. for determination in 7-8 rats/group from corresponding tissue punches. Data analyzed by two-way ANOVA and differences between all the groups tested by Newman-Keuls analysis. * group different from all other groups, at a level of p 0.05. VDB, vertical limb of the diagonal band; HDB, horizontal limb of the diagonal band; vGP, ventral part of the globus pallidus.