In: Basal Forebrain: Anatomy to
Function,
C.T. Napier, P.W. Kalivas and I.
Hanin,
eds., Plenum, New York, 1991 pp.
43-100.
AFFERENTS TO BASAL FOREBRAIN
CHOLINERGIC PROJECTION NEURONS: AN UPDATE
Lászlo Záborszky1, William E. Cullinan1
and Alex Braun2
1Departments of Otolaryngology,
Neurosurgery and Neurology
University of Virginia Health
Science Center, Charlottesville, VA 22908
2Department of Pathology, State
University of New York at Stony Brook, New York 11780
The
basal forebrain cholinergic projection (BFC) system has been the focus of
considerable attention as a result of evidence implicating it in a number of
behavioral functions, including arousal, sensory processing, motivation,
emotion, learning, and memory (Deutsch, l983; Buzsaki et al., l988; Richardson
and DeLong, 1988; Durkin, l989; Rolls l989, Steriade and McCarley, l990).
Moreover, neuropathological changes in the BFC have been reported in a
surprisingly large number of neurological diseases, including Alzheimer's and Parkinson's
diseases (for ref. see Coyle et al., l983; Mesulam and Geula, l988; Arendt et
al., l989). BFC neurons in the rat are dispersed across a number of classically
defined territories of the basal forebrain, as illustrated from a series of
coronal sections in Fig. 1. However, using a computer graphic three-dimensional
reconstruction technique (Schwaber et al., 1987) or manual reconstruction from
camera lucida drawings (Fig. 2) it is evident that BFC neurons form a
continuum, rather than being arranged as distinct nuclear groups.
Although
there is considerable species variation in the precise locations of cholinergic
projection neurons in the basal forebrain, the efferent projections of these
cells follow basic organizational principles in all vertebrate species studied.
Thus, neurons within the medial septum and nucleus of the vertical limb of the
diagonal band (MS/VDB; also termed Ch1/Ch2 according to the classification of
Mesulam et al., 1983b) provide the major cholinergic innervation of the hippocampus;
cholinergic cells within the horizontal limb of the diagonal band and magnocellular preoptic nucleus (HDB/MCP;
Ch3) project to the olfactory bulb, piriform, and entorhinal cortices;
cholinergic neurons located in the ventral pallidum, sublenticular substantia
innominata (SI), globus pallidus, internal capsule, and nucleus ansa
lenticularis, collectively termed the nucleus basalis (Ch4), project to the basolateral amygdala, and innervate the
entire neocortex according to a rough medio-lateral and antero-posterior
topography (Sofroniew et al., 1982; Armstrong et al., 1983; Mesulam et al.,
l983a,b; Lamour et al., l984b; Rye et al., l984; Woolf et al., l984; Amaral and
Kurz, l985; Carlsen et al., l985; Wainer et al., l985; Woolf et al., l986;
Záborszky et al., 1986a; Luiten et al., l987; Nyakas et al., l987; Sofroniew et
al., l987; Fisher et al., l988; Gaykema et al., 1990). In the primate the corticopetal cholinergic
cells (Mesulam et al., 1983b; Mesulam
et al., 1986a; see also Butcher and Semba, 1989; Heimer et al., 1991a) are
subdivided according to the topography of their projections. Thus, the
anteromedial sector (Ch4am) projects to medial cortical areas including the
cingulate gyrus; the anterolateral compartment (Ch4al) to ventral orbital, frontal,
and parietal opercular regions, as well as the amygdala; the intermediate
sector (Ch4i) to lateral frontal, parietal, peristriate and temporal regions
including the insula; and the posterior sector (Ch4p) to superior temporal and
temporopolar areas (Mesulam et al., l983a, l986a; Mesulam and Geula, l988).
Despite
the wealth of data on the topography of the efferent projections of BFC
neurons, information concerning the afferent connections of these cells has
been more elusive. The reasons for this are easily appreciated in view of the
anatomical complexity of the basal forebrain, in which cholinnergic neurons are
intermingled among numerous non-cholinergic cells (Záborszky et al., l986a;
Walker et al., 1989), and are distributed in close proximity to several major
ascending and descending fiber systems (for review see Záborszky, l989a).
Therefore the verification of actual synaptic contact between the afferent
fiber systems and the cholinergic projection neurons requires appropriate
combinations of double immunocytochemical methods at the ultrastructural level,
in which the afferent system and the cholinergic nature of postsynaptic target
can be unequivocally determined (Záborszky and Heimer, l989). The study of
these inputs is further complicated by the morphological characteristics of
these neurons. For example, several studies suggested that the dendrites of BFC
neurons extend for very long distances (Semba et al., l987; Brauer et al.,
1988; Dinopoulos et al., 1988), and our own experiments involving combinations
of Golgi labeling and choline acetyltransferase (ChAT) immunocytochemistry
(Fig. 3) have confirmed this point. Also, synaptic input to the cell bodies and
proximal dendrites of these cells is notably sparse, although it increases on
more distal dendritic segments (Ingham et al., l985; Dinopoulos et al., 1986).
Thus, a complete characterization of the afferents of the BFC cells will need
to take these factors into account. This information is prerequisite to the
design of pharmacological and behavioral investigations of BFC function.
This
review will survey data regarding afferents to the basal forebrain with special
reference to potential inputs to BFC neurons, including their transmitters, as
well as summarize the current state of knowledge from ultrastructural studies
of identified afferents to these cells. Finally, we will discuss some possible
functional roles of BFC neurons in behaviors as they may relate to specific
neural circuits.
Studies
in monkeys and cats (Mesulam and Mufson, 1984; Irle and Markowitsch, l986) are
consistent with the notion that basal forebrain areas rich in cholinergic
neurons receive only a restricted cortical projection, originating from so
called paralimbic cortical areas (Mesulam et al., l986b), including the
orbitofrontal cortex, the anterior insular cortex, temporal polar, medial
inferotemporal region, entorhinal, piriform, and perirhinal cortices. BFC
neurons do not appear to receive direct input from primary sensory and motor
cortex or from most higher order association areas. In contrast, in a study in
the rat involving wheat germ agglutinin-horseradish peroxidase (WGA-HRP)
injections into different cortical areas (Saper, l984), it was suggested that
cortical projections to BFC neurons originate in widespread cortical areas, and
that these projections are reciprocal in nature to the corticopetal projection
of the BFC system. However, data from recent studies in the rat, in which
retrograde tracers were delivered to different regions of the BFC system, do
not support this concept (Lamour et al., 1984b; Haring and Wang, l986; Grove, l988; Jones and Cuello, l989; Semba
and Fibiger, l989; Carnes et al., l990), but rather suggest a restricted origin
of cortical input to basal forebrain areas containing cholinergic neurons in
rodents, similar to primates. Indeed, our preliminary light microscopic
experiments in the rat, involving anterograde tracing with Phaseolus vulgaris leucoagglutinin (PHA-L) from different cortical
areas (Cullinan and Záborszky, in preparation), are consistent with the notion
that cortical inputs to the BFC are restricted to a number of allocortical
regions, including orbital areas,
insular cortex, and perhaps the piriform cortex. One reason for the discrepancy
with the results of Saper (l984) appears to be that many corticofugal axons of
neocortical origin descending in the internal capsule, although located in
close proximity to corticopetal cholinergic neurons, are in most instances
smooth and devoid of varicosities, while axons from allocortical regions showed
profuse terminal arborizations in some portions of the basal forebrain, where
they could be seen to approximate cholinergic neuronal elements in patterns suggestive
of synaptic contact.
In
the rat, the pattern of input to the basal forebrain from the various
allocortical regions investigated appears to be restricted. For example,
terminal arborizations of orbitofrontal axons were found mainly in the ventral
portions of the MCP and HDB (Fig. 4). Interestingly, the forebrain regions
which receive inputs from allocortical areas correspond well with the location
of BFC neurons projecting to the same cortical areas, suggesting a reciprocity
between BFC neurons and allocortical areas.
Although
the possibility cannot be excluded that a proportion of the cholinergic neurons
receiving allocortical input project to
cortical
regions, other than allocortex, it is clear that
large portions of the cortex are not influenced from the allocortex
via BFC cells. This is
particularly true for many neocortical areas (e.g.
sensorimotor, auditory), which receive cholinergic projections from BFC neurons
located primarily in the internal capsule, globus pallidus, and peripallidal
regions, areas which are apparently not reached by projections from
allocortical regions. While it has been suggested that in the primate
paralimbic cortical areas may influence not only their own cholinergic
innervation, but also that of wide-spread cortical regions by virtue of their
projections to the BFC (Mesulam and Mufson, l984), these conclusions were based
on autoradiographic data, in which fibers of passage are not clearly
distinguished from terminals. Therefore, the extent to which paralimbic
cortical afferents might control the BFC system in the primate remains to be
disclosed. On the other hand, cortical areas may indirectly influence their own
cholinergic input through the striatopallidal system (see below), or through
cortico-cortical connections.
The
behavioral specializations of the orbitofrontal cortex are remarkably similar
in rodents and primates, including humans, and are thought to include the
regulation of autonomic functions, feeding, species-specific social-affective
behaviors, motivation, and some aspects of learning and memory (Kolb, l984;
Goldman-Rakic, l987; Fuster, l989; Sesack et al., 1989). Monkeys with
orbitofrontal lesions were particularly impaired on behavioral tasks requiring
the frequent making and breaking of stimulus-reward associations (Jones and
Mishkin, 1972). In the absence of the orbitofrontal cortex, these animals
continue to respond to inappropriate (unrewarded) stimuli, similar to humans
with frontal lobectomy.
Electrophysiological
experiments in primates have revealed that basal forebrain, possibly
cholinergic neurons, similar to many orbitofrontal units, respond to a range of
visual and auditory stimuli that have been reinforced (Richardson and De Long,
l988; Rolls, l989; Wilson and Rolls, l990a,b). It has been suggested that this
information is conveyed to the basal forebrain through the orbitofrontal cortex
(Rolls, l989).
Behavioral
studies in primates have shown that the dorsolateral prefrontal cortex, or in
rats the medial prefrontal cortex, is critically concerned with an animal's ability
to connect events across time, as ablations of this region result in severe
deficits in performance on delay tasks (Kolb, l984; Goldman-Rakic, 1987;
Fuster, l989). Electrophysiological studies in behaving monkeys revealed that
prefrontal units which participate in different phases of behavior organization
show a topographical distribution. Units encoding the sensory cue (both its
specific sensory nature and its behavioral significance), are located rostrally
in the prefrontal cortex; other units, which discharge in relation to the
anticipatory motor action, or engaging in the rapid relay between the first two
categories of units, are located more caudally towards the premotor and motor
areas. Finally, units which are related to reward or punishment concentrate in
orbital areas and are intermingled with units responding to gustatory and
olfactory inputs (Rosenkilde et al., 1981; Fuster et al., 1982; Thorpe et al.,
l983; Innoue et al., 1985). Although the exact mechanism by which these
different units participate in the temporal organization of behavior is
unclear, the cholinergic system, with afferents from the orbitofrontal cortex
and probable projections back to the prefrontal cortex, is likely to influence
these processes. Since corticofugal projections are thought to be primarily
excitatory (Fonnum et al., l981; Giuffrida and Rustioni, l988), and a
facilitatory role has been typically ascribed to acetylcholine (ACh) from
physiological studies, feedback through the BFC system could conceivably hold
the information about previously experienced stimuli current or 'on line' in
prefrontal-cortical circuits over the delay period (while the stimulus is
absent) to guide appropriate responses. This notion is consistent with the
results of pharmacological and lesion studies, as well as neuropsychiatric
data, indicating that disruption of the BFC system is associated with deficits
in memory functions (Drachman and Leavitt, 1974; Bartus et al., 1982).
The
nucleus accumbens, together with the ventral part of the caudate putamen, the
olfactory tubercle, and the striatal cell bridges that connect these
structures, form the ventral striatum (Heimer and Wilson, l975). A major output
of the ventral striatum is directed to the ventral extension of the globus
pallidus known as the ventral pallidum, in a topographically organized manner
(Heimer and Wilson, l975; Nauta et al., l978; Mogenson et al., l983; Haber et
al., l990; Parent, l990; Heimer et al., l991a,b).
In rat, many cholinergic
projection neurons invade the ventral pallidum and dorsal pallidum (or globus
pallidus), or the narrow spaces between fiber bundles of the internal capsule,
and it was suggested based upon light microscopic data (Grove et al., l986)
that cholinergic neurons share to some extent the afferents of neighboring
noncholinergic pallidal neurons. In experiments using PHA-L tracing and ChAT
immunocytochemistry at the ultrastructural level (Cullinan and Záborszky, in
preparation) we confirmed our preliminary electron microscopic degeneration
data (Záborszky et al., l984b; Fig. 5) that nucleus accumbens axons establish
synaptic contact with ventral pallidal cholinergic neurons. Since the majority
of the PHA-L labeled neurons at the injection site in the nucleus accumbens in
our study were also immunopositive for calcium binding protein (calbindin D28),
a marker for a subpopulation of GABAergic neurons in this region, it is likely
that the neurotransmitter involved in this projection is, in large part, GABA.
Indeed, terminals containing the enzyme glutamic acid decarboxylase (GAD), the
GABA synthesizing enzyme, have been confirmed to terminate on cholinergic
neurons in the ventral pallidum (Záborszky et al., l986b). Substance P and
enkephalin are also transmitter candidates for these inputs, as lesions of the
nucleus accumbens result in decreases in immunostaining for these substances in
the ventral pallidum (Walaas and Fonnum, 1979; Záborszky et al., l982; Haber
and Nauta, l983), and substance P and enkephalin containing terminals have been
found to contact cholinergic neurons in pallidal regions (Bolam et al., l986;
Martinez-Murillo et al., l988a). These substances may be co-localized with
GABA, since striatal neurons have been shown to co-localize GABA and either
substance P or enkephalin (Penny et al., l986).
In
autoradiographic (Mesulam and Mufson, l984; Haber et al., l990) and limited
PHA-L tracing experiments in primates (Haber et al., l990) it has been
suggested that axons originating from the nucleus accumbens and ventromedial
portions of the caudate and putamen innervate the nucleus basalis. The extent
to which these fibers terminate in this region or merely pass through, however,
remains to be more fully disclosed.
The
possibility that cholinergic neurons receive ventral striatal input in
primates, together with the observations of a topographical organization within
corticostriatal projections (Phillipson and Griffiths, l985; Goldman-Rakic and
Selemon, l986; McGeorge and Faull, 1989), ventral striatal projections (Haber
et al., l990; Heimer et al., 1991b), and in the BFC system, may be of interest
in the light of recent ideas about the functional organization of forebrain
circuits that involve the cortex and basal ganglia. Alexander et al. (l986)
have suggested that in primates distinct regions of the frontal cortex, basal
ganglia, and the thalamus are connected through parallel, functionally
segregated circuits. Cholinergic neurons receiving ventral striatal input may
be part of such channels, however these would provide an extrathalamic outflow
to the cortex. Several of such distinct circuits can be envisaged (Fig. 6,
upper row), although more detailed data are needed, particularly with respect
to the input-output relations of identified BFC neurons. Since the
corticostriatal as well as the hippocampoor amygdalofugal projections are most
probably excitatory (Fonnum et al., 1981; Christie et al., 1987; Fuller et al.,
l987), and the input from the ventral striatum to the cholinergic neurons is
likely to be inhibitory, the activation of this corticostriatal link would
appear to result in reduced cholinergic activity in the cortical target
areas.
In rat,
medial and lateral parts of the prefrontal cortex, (anterior cingulate, prelimbic,
agranular insular) the perirhinal, entorhinal cortices, the amygdala and the
hippocampus project to the ventral striatum in a topographical fashion
(Krayniak et al., 1981; Kelley and Domesick, 1982; Kelley et al., l982;
Phillipson and Griffith, l985; Swanson and Köhler, l986; Fuller et al., l987;
Groenewegen et al., l987; Sesack et al., l989; Witter et al., l989; Groenewegen
et al., 1990), and the projections from the ventral striatum to the pallidal
complex also maintain a high degree of topographical organization (Gerfen,
l985; Heimer et al., 1991a,b). Cholin-nergic neurons of the ventral pallidum
project to the basolateral amygdala (Carlsen et al., l985), and to the medial
prefrontal cortex and an adjoining medial strip of the motor cortex as well as
allo(piriform and entor-hinal cortex) and periallocortical (insula) regions
(Saper, l984; Haber et al., l985; Luiten et al., l987). Thus it appears that
cholinergic neurons within the ventral pallidum may also participate in
cortico-striatopallido-cortical loops (Fig. 6, lower row). In view of the fact
that afferents from different cortical areas and the amygdala project to the
striatum in a complicated, often overlapping or interdigitating fashion
(Groenewegen et al., l990) in rats, similar to monkeys (Selemon and
Goldman-Rakic, l985), and since the topography of the cholinergic projection
neurons in the VP is not well understood, two-chain tracing/immunocytochemical
experiments are required to determine whether such pathways involving cholinergic
projections exist as proposed in Fig. 6.
The
significance of striatal input to pallidal cholinergic neurons would appear to
vary according to the species. In primates, only a small number of cholinergic
cells are located in the internal and external medullary laminae of the globus
pallidus, or are embedded within the fibers of the internal capsule, in
addition to the few neurons found within the globus pallidus proper (Mesulam et
al., l983a; Saper and Chelimsky, l984; Everitt et al., 1988; Mufson et al.,
l989). It is not clear, however, whether these neurons are reached by
striatofugal fibers from the dorsal striatum, or only by aberrant fibers from
the stria terminalis (Price and Amaral, l981; Price et al., l987). In contrast,
in rat, a considerable population of corticopetal cholinergic neurons are
located in the globus pallidus, internal capsule and peripallidal areas, and it
has been suggested that cholinergic neurons in these areas receive striatal
input from the medial striatum (Grove et al., l986; Shu et al., 1988). In rat,
corticostriatal projections from prefrontal, auditory, visual cortices,
hippocampus and the amygdala project to the medial part of the dorsal striatum
in a topographical manner (e.g. Beckstead, l979; McGeorge and Faull, l989). Due
to the complex compartmental terminations of the different afferents in the
dorsal striatum (e.g. Gerfen, 1984; Donoghue and Herkenham, 1986; Faull et al.,
l986; Gerfen, l989), it is unclear which afferents might reach those striatal
cells, which in turn, project to cholinergic neurons in the globus pallidus.
In
summary, striatal afferents to BFC neurons may constitute a way by which
different cortical regions indirectly control the cholinergic input they
receive. Future studies should identify the specific neuronal elements involved
in these circuits, and determine how this indirect cortical control may be
related to the more direct cortical control of cholinergic function.
The
majority of hippocampal projections to the septum in rat (Swanson and Cowan,
l979; Groenewegen et al., 1987) course through the fimbria-fornix and terminate
on spiny multipolar neurons (Alonso and Frotscher, l989) and GABAergic neurons
(Leranth and Frotscher, l989) in the lateral septal nucleus. It has been
suggested that lateral septal neurons establish synaptic contacts with
cholinergic and GABAergic neurons in the medial septum which in turn project
back to the hippocampus, but that hippocampopetal neurons in the MS/VDB do not
receive afferents from the hippocampus directly (Köhler et al., l984; Freund
and Antal, l988; Leranth and Frotscher, l989). Light microscopic tracing
studies in primates (Aggleton et al., l987) and rodents (Carnes et al., l990)
suggested that hippocampal efferents en route to the lateral hypothalamus and
amygdala may terminate on neurons in the MS/VDB complex and medial part of the
SI, however, the notion that cholinnergic hippocampopetal neurons receive
direct input from the hippocampus remains questionable and requires
confirmation.
Autoradiographic
studies in primates and rodents have shown that fibers originating from
different amygdaloid nuclei course through the HDB and SI as part of the
ventral amygdalofugal pathway en route to other brain regions, including the
hypothalamus, thalamus, striatum, and prefrontal cortical areas (Krettek and
Price, l978; Price and Amaral, 1981; Russchen et al., l985a,b; de Olmos, 1990).
Although the extent to which these projections terminate in the SI was unclear
due to the limitations of the autoradiographic method, experiments using the
PHA-L technique have shown that amygdaloid fibers have varicosities along their
trajectory through the SI (Russchen and Price, l984; Russchen et al., l985a).
In double-labeling experiments at the electron microscopic (EM) level in the
rat, we have shown that fibers from the basolateral amygdaloid nucleus form
synapses on dendrites of both cholinergic and noncholinergic cells in the
ventral pallidum (Záborszky et al., 1984a).
The extent to which BFC neurons outside of the ventral pallidum receive
amygdaloid afferents remains to be determined, as do the projection targets of
those cells which may receive this input. The available evidence, however,
suggests that the amygdaloid complex as
a whole is reciprocally connected with the BFC system in both rat and primate,
but that this reciprocity is not exact.
Neurons
of the amygdala receive specific multimodal sensory information (Aggleton et
al., l980; Turner et al., 1980; van Hoesen, l981; Ruschen et al., l985a,b;
Amaral, l987; LeDoux, l987) and participate in stimulus-reward-associative
learning (Gaffan and Harrison, 1987; Nishijo et al., 1988; LeDoux et al.,
l990). It is likely that a subpopulation
of BFC neurons participate in similar processes through amygdaloid input.
It
is interesting to note that polysensory paralimbic cortical areas, including
the temporal pole, inferotemporal, orbitofrontal, perirhinal and entorhinal
cortices, which project to the
amygdaloid nuclei, also project to the SI in primates (Aggleton et al., l980;
Turner et al., 1980; van Hoesen, l981; Russchen et al., 1985a,b). Similar
interconnections are also known in rat (Witter et al., l989; Groenewegen et
al., l990). Therefore, from a functional point of view, it will be important to
examine the possible convergence of cortical and amygdaloid inputs to the same cholinergic neuron.
Based
upon hodological and chemoarchitectural characteristics, certain parts of the amygdala,
the SI, and bed nucleus of the stria terminalis (BSt) constitute a
morphological and perhaps functional entity, as originally suggested by de
Olmos et al. (l985), and discussed recently in several papers (Alheid and
Heimer, l988; Moga et al., l990; Heimer et al., l991a; Heimer and Alheid, this
volume). In particular, the lateral BSt-SI-central nucleus of the amygdala
share many connections. Similarly, an affiliation between the medial segment of
the BSt and medial nucleus of the amygdala and an intervening portion of the SI
has been suggested. The results of a PHA-L tracing study (Grove, 1988) have
suggested that the lateral BSt and the central amygdala project to the more
dorsal SI/ventral part of globus pallidus, while the medial BSt, medial and basomedial
amygdaloid nuclei project to more ventral parts of the SI. Cholinergic
projection neurons located within these subdivisions may thus subserve
different functional roles accordingly.
The
possibility of hypothalamic input to the general forebrain regions containing
cholinergic projection neurons was suggested from a number of autoradiographic
studies (Conrad and Pfaff, l976a,b; Saper et al., l976; Swanson, l976; Saper et
al., 1978; Krieger et al., l979; Saper et al., l979; Berk and Finkelstein,
l982; Mesulam and Mufson, l984; Saper, 1985), although this issue remained
somewhat unclear due to the difficulty in distinguishing fibers from terminals
with the autoradiographic technique. More recent experiments using the anterograde
tracer PHA-L have confirmed the presence of projections to these regions from
several hypothalamic nuclei (ter Horst and Luiten, l986; Simerly and Swanson,
1988) and a light microscopic double-labeling study investigated the
distribution of hypothalamic afferents to cholinergic cells in the SI (Grove,
l988). We recently have mapped the
distribution of hypothalamic axons originating from various portions of the
caudal lateral hypothalamus and from different medial
hypothalamic cell groups in relation to the BFC system in its entirety
(Cullinan and Záborszky, l991). Inputs to the cholinergic projection system
were distributed in a manner reflecting the gross topography of the ascending
hypothalamic projections. Axons originating from neurons in the far-lateral
hypothalamus reach cholinergic neurons in a zone which extends from the dorsal
part of the SI caudolaterally, to the lateral portion of the bed nucleus of the
stria terminalis rostromedially, encompassing a narrow band along the ventral
part of the globus pallidus and medial portion of the internal capsule. Axons
originating from cells in the more medial portions of the lateral hypothalamus
reach cholinergic cells primarily in more medial and ventral parts of SI, and
in the magnocellular preoptic nucleus and HDB. Finally, axons from medial
hypothalamic cells, particularly the anterior hypothalamic and medial preoptic
areas, appear to contact cholinergic neurons primarily in the medial part of
HDB, and in the MS/VDB complex (Fig. 15D-F). Electron microscopic
double-labeling experiments confirmed contacts between labeled terminals and
cholinergic neurons in the HDB and SI, both from the lateral and medial
hypothalamus (Záborszky and Cullinan, l989, Cullinan and Záborszky, l991; Fig.
7). A comparison of the topography of corticopetal and hippocampopetal neurons
(Mesulam et al., l983a; Rye et al., l984; Woolf et al., l984; Amaral and Kurz,
l985; Woolf et al., l986) with our findings suggests that corticopetal
cholinergic neurons are innervated by lateral hypothalamic neurons, while
hippocampopetal cholinergic neurons located largely within the MS/VDB complex
are likely to receive input mainly from medial hypothalamic groups.
Considering the fact that lateral hypothalamic neurons receive both
general and special viscerosensory information (Fulwiller and Saper, l984)
either directly from the nucleus of the solitary tract or through the parabrachial
nucleus, it is possible that this integrated viscerosensory input reaches BFC
neurons through lateral hypothalamic projections. The significance of afferents
from the medial hypothalamus is less clear, as this region is thought to
participate in the control of a number of neuroendocrine, autonomic, and
behavioral mechanisms (Záborszky, l982; Swanson, 1987). A recent review
(McGinty and Szymusiak, l990) suggested that inhibitory input from preoptic neurons to basal
forebrain cholinergic neurons could be
involved in thermoregulatory and hypnogenic mechanisms. Interestingly, in our
combined electron microscopic study (Cullinan and Záborszky, l991), we
have identified symmetric synaptic
contacts on cholinergic neurons in the basal forebrain originating from the
medial preoptic-anterior hypothalamic area, suggesting an inhibitory influence.
Studies
in the rat involving the delivery of retrograde tracers into the SI/globus
pallidus area resulted in labeling of neurons within the parafascicular nucleus
(Grove, l988; Jones and Cuello, l989; Carnes et al., l990), while injections
into more caudoventral parts of the SI produced retrograde labeling in the
paratenial, paraventricular, central medial, rhomboid and reuniens thalamic
nuclei (Grove, l988; Carnes et al., l990). The interpretation of these results
is confounded by technical difficulties, however, since these tracers often
involve uptake by fibers of passage, and axons from intralaminar and medial
thalamic nuclei, as components of the inferior thalamic radiation, pass through
the internal capsule and globus pallidus en route to the striatum and cortex
(Herkenham, l978, l979; Beckstead, 1984; Kelley and Stinus, l984; Royce and
Mourey, l985).
Using
the autoradiographic tracing method, Ricardo (l981) described a significant
projection from the zona incerta to several rostral forebrain structures
including the SI, ventral pallidum, and globus pallidus. Since the labeling in
these structures appeared to represent an end-point, it is likely that many
fibers actually establish synaptic contact in these regions. Both the zona
incerta and the intralaminar thalamic nuclei are known to receive significant
projections originating from several levels of the brainstem reticular
formation, to project upon widespread regions of the cortex (Macchi and
Bentivoglio, l986), and have been implicated in the modulation of cortical
activity (Steriade and McCarley, l990). In view of evidence implicating the BFC
system in arousal (see below), it will be important to determine whether
brainstem reticular influences upon the cortex are also mediated through a
subthalamic relay to the BFC system.
Following
knife cuts at the meso-diencephalic border, degenerating terminals were found
in synaptic contact with BFC neurons (Fig. 8A,B), and this intervention also
resulted in a decrease of ChAT (choline acetyltransferase) activity in
forebrain areas rich in cholinergic cell bodies (Záborszky et al., l986c).
Although this preliminary study did not reveal the origin or chemical
specificity of afferents, it was the first direct evidence of brainstem input
to BFC neurons, and further suggested that removal of brainstem afferents may
induce transynaptic changes in these cells. Since this report we have confirmed
the presence of locus coeruleus terminals on BFC neurons. Several other
brainstem afferent systems are also potentially important, since they might
mediate the effects of reticular activation upon the cortex.
Peripeduncular Area
The
peripeduncular area (PPa) is located ventrally and medially to the anterior
part of the medial geniculate body (MGB), abutting the dorsal end of the
cerebral peduncle and the substantia nigra pars lateralis. It contains several
cell groups such as the peripeduncular nucleus (PP) and the posterior
intralaminar nucleus, which as parts of the "adjunct" auditory system
receive acoustic signals from different auditory relay stations, although with
less topographic and modality specificity (LeDoux et al., l985; Arnault and
Roger, l987; LeDoux et al., 1990). Using
the autoradiographic tracing method in primates, Jones et al. (l976)
suggested that the PP projects to the large aggregated neurons of the SI and
medullary laminae of the globus pallidus. In the rat, retrograde tracing
experiments (Jones and Cuello, l989), as well as anterograde tracing studies
using PHA-L (Grove, l988), have confirmed that neurons located in the PPa
project rather specifically to the vicinity of BFC neurons in the caudal part
of the globus pallidus, adjacent to the internal capsule. Since the same group
of cholinergic neurons which are likely to receive input from the PPa have
been shown to project to the auditory
cortex (Rye et al., l984), the PPa may provide a relay through which a
subpopulation of cholinergic neurons participates in auditory sensory
processing (Fig. 9A).
Parabrachial Nucleus
The
brachium conjunctivum is surrounded by several cytoarchitectonically distinct
cell groups along its course through the dorsolateral pons (Fulwiler and Saper,
l984). Both the medial and lateral nuclei have widespread ascending projections
innervating different hypothalamic, amygdaloid and thalamic nuclei, basal
forebrain and cortical areas (Saper and Loewy, 1980; Fulwiller and Saper, l984;
Semba et al., l988; Vertes, 1988; Jones and Cuello, 1989). Following PHA-L injections into the lateral
segment of the parabrachial nucleus (PB), varicose fibers were evident
throughout the dorsal part of SI, lateral BSt, and lateral part of the central
nucleus of the amygdala (Grove, 1988).
The
parabrachial nucleus relays both specific (gustatory) and general (respiratory,
cardiovascular, gastrointestinal) viscerosensory input from the nucleus of the
solitary tract (NTS) to the viscerosensory nuclei of the thalamus, and the
insular cortex (Saper, l982; Block and Schwartzbaum, l983; Cechetto and Saper,
l987; Ruggiero et al., l987). Cholinergic cells in the SI may receive specific
viscerosensory input through the medial PB, since it is this region of the PB
that receives afferents from the rostral, gustatory part of the NTS (Norgren,
l978), and is where many of the labeled cells were found following injections
of retrograde tracers in the SI (Fullwiller and Saper, l984). However, BFC
neurons in the SI may also receive general viscerosensory input from the
lateral PB, since the lateral PB receives input from the caudomedial, general
viscerosensory portion of the NTS (Ricardo and Koh, 1978; Milner et al., l984),
and this portion of the parabrachial nucleus contained retrogradely labeled
cells backfilled from the SI (Fullwiller and Saper, 1984; Moga et al., l990).
Interestingly, neurons in the SI also appear to receive direct input from the
caudal NTS area, therefore cholinergic neurons may receive general
viscerosensory input both directly, and to some extent indirectly through the
PB, but are likely to receive gustatory input mainly indirectly through the PB
relay.
Information
relayed through the BFC system from the PB is likely to reach, among other
cortical regions, the insular cortex. The insular cortex receives highly processed viscerosensory
information from the thalamus (Cechetto and Saper, l987). Viscerosensory
information relayed through the BFC system to the insular cortex might thus
provide a primitive representation of the stimulus. These relationships are
illustrated in the simplified circuit diagram of Fig. 9. It should be added
that the PB and caudal NTS also project to the central nucleus of the amygdala
(Saper, l982; Shipley and Geinisman, l984; de Olmos, 1985), which is itself a
likely source of input to BFC neurons in the SI. Thus, a certain population of
cholinergic neurons in the SI might participate in a complex neural circuit
implicated in viscerosensory information processing, conditioned taste
aversion, (Lasiter et al., l985) and affective evaluation of the stimulus
(LeDoux, 1987). According to recent studies, information from the NTS is
relayed through the PB in a much
more complicated fashion (Herbert et
al., l990; Moga et al., 1990). It will be a challange for future studies to
identify whether cholinergic cells indeed participate in such circuits and if
so what type of specific information they receive.
The
pedunculopontine tegmental nucleus (PPT) and the laterodorsal tegmental nucleus
(LDT) in the mesopontine tegmentum represent the primary sources of cholinergic
projections to the thalamus (Sofroniew et al., 1985; Levey et al., l987; Pare
et al., l988; Steriade et al., l988). In addition, a cholinergic projection to
the medial prefrontal cortex from the LDT has been described (Satoh and
Fibiger, l986), and it has been suggested that a subpopulation of cholinergic
neurons, mainly in the magnocellular preoptic nucleus (MCP), may receive
cholinergic input from the LDT/PPT area (Woolf and Butcher, 1986; Satoh and
Fibiger, l986; Semba et al., l988; Jones and Cuello, 1989).
The
majority of neurons in these areas that project to the thalamus display tonic
discharge patterns and increment firing rates that precede the earliest change
from EEG synchronization during quiet sleep to EEG desynchronization during REM sleep (Steriade and McCarley, l990).
The PPT/LDT may thus be considered the best candidate for inducing EEG
desynchronization, which parallels increased ACh release (Kanai and Szerb,
l965; Jasper and Tessier, l971). A cholinergic/cholinergic interaction in the
basal forebrain would also be congruent with the original concept that
stimulation of the midbrain reticular formation could evoke desynchronization
of the EEG (Moruzzi and Magoun, 1949). Despite intensive efforts, however,
there is no convincing evidence for a significant cholinergic projection to BFC
neurons (see also Hallanger and Wainer, l988). In contrast, a preliminary
electron microscopic study has suggested that the ascending cholinergic axons
from the mesopontine tegmentum establish synaptic contact with non-cholinergic
neurons of the basal forebrain (Hallanger et al., l988).
Ventral Tegmental Area-Substantia Nigra-Retrorubral
Field
Using
autoradiographic tracing from the dopaminergic ventral tegmental area (VTA),
substantia nigra (SN) and retrorubral field (RRF), labeled fibers were traced
through basal forebrain areas rich in cholinergic cells (Fallon and Moore,
l978; Beckstead, 1979; Simon et al., l979; Vertes, l988). Retrograde tracing
studies have confirmed the presence of labeled cells primarily in the VTA after
tracer injections into the VDB/HDB area, while tracer injections into the
cholinergic rich region of the globus pallidus (Haring and Wang, 1986;
Hallanger and Wainer, l988;
Martinez-Murillo et al., 1988b; Semba et al., 1988; Jones and Cuello,
l989) resulted in a large number of retrogradely labeled cells in the SN and
RRF. In the substantia nigra the majority of the cells were in the zona
compacta (SNc), with few seen in the pars reticulata. The simultaneous
detection of retrogradely labeled cells and dopaminergic neurons using an
antibody against tyrosine hydroxylase (TH) revealed that almost all retrograde
neurons in the SNc were double labeled, while in the VTA and RRF 75-82% of the
retrogradely labeled cells contained TH (Jones and Cuello, l989). Since many
dopaminergic axons in the globus pallidus and other forebrain areas represent
fibers of passage toward the striatum, septum, and cortex, further studies are
needed to determine the origin and distribution of putative dopaminergic input
to BFC neurons.
Locus Coeruleus
Evidence
from autoradiographic and retrograde tracing studies has suggested projections
to forebrain areas rich in cholinergic projection neurons from the locus
coeruleus (Semba et al., 1988; Jones and Cuello, 1989). However, since axons
from the locus coeruleus are known to pass through the basal forebrain areas en
route to the cortex (Jones and Moore, l977; Jones and Yang, l985), these data
are of limited value in determining the extent to which these projections
terminate in the basal forebrain and contact BFC neurons. In a PHA-L study
(Záborszky et al., in preparation) in which the majority of the labeled cells
at the injection site were localized within the locus coeruleus, labeled
varicosities were detected in direct apposition to cholinergic projection
neurons in extensive basal forebrain areas, including the MS/VDB complex, HDB
and SI. In contrast, cholinergic neurons in peripallidal regions appear to
receive very few contacts. Results of experiments at the electron microscopic
level indicate that cholinergic neurons are indeed contacted by locus coeruleus
axons, and that an individual locus axon can establish synaptic contacts with
both cholinergic and non-cholinergic neuronal elements (Záborszky et al., in
preparation).
Locus
coeruleus neurons are activated by a wide variety of afferent stimuli, including
auditory, visual, somato and viscerosensory (Aston-Jones and Bloom, l981; Foote
et al., l983), as well as stressful and/or aversive stimuli (Abercrombie and
Jacobs, l987, Jacobs, l987), although there is controversy concerning whether
this nucleus receives afferent information from widespread (Cederbaum and
Aghajanian, l978) or restricted (Aston-Jones et al., l986) areas. Locus
coeruleus cells, similar to dorsal raphe serotonergic neurons, show
pacemaker-like activity and state-related modulation of discharge, which is
highest in the waking state and decreases in slow wave sleep. Arousing stimuli
are accompanied by bursting activity of these neurons (Steriade and McCarley,
l990). Electrophysiological studies have shown that noradrenergic activation
often leads to enhanced efficacy of synaptic transmission for other afferents
that converge upon the same postsynaptic neuron (Foote et al., l983; Björklund
and Lindvall, l986). Locus coeruleus axons may therefore participate in
selective attention by filtering out irrelevant stimuli and thus increase the
signal-to-noise ratio of behaviorally significant stimuli. In view of the fact
that similar physiological mechanisms have been described generally for the
action of acetylcholine on its target neurons, as well as morphological
evidence that locus coeruleus axons terminate on BFC neurons, these effects may
be mediated both indirectly through the connections of the locus coeruleus with
the BFC system, as well as directly through its widespread corticopetal projections.
An important difference between BFC and locus coeruleus mediated arousal is
that locus coeruleus neurons virtually cease discharge with the advent of REM
sleep (Steriade and McCarley l990).
Phasic
activation of cholinergic neurons by noradrenergic afferents has been
implicated in certain memory processes (Durkin, l989), which is compatible with
the observation that LC neuronal
activity is increased during conditioning (Jacobs, l987).
Raphe Nuclei
The
results of retrograde tracing experiments have suggested projections from the
raphe nuclei to areas containing BFC neurons (Semba et al., l988; Vertes, 1988;
Jones and Cuello, l989; Carnes et al., 1990). Consistent with data from
autoradiographic studies (Azmitia and Segal, l978; Vertes and Martin, l988), it
appears that the MS/VDB complex receives projections mainly from the median
raphe nucleus (MR; B8 serotonergic cell group of Dahlström and Fuxe, l964),
while inputs to the MCP/HDB and SI/globus pallidus regions originate from the
dorsal raphe nucleus (DR; B7 cell group). Additional projections have been
described from the caudal linear, interfascicular, magnus, pontine raphe
nuclei, and from neurons within the B9 serotonergic cell group in the
mesopontine ventral tegmentum (Molliver, l987; Semba et al., 1988; Vertes,
l988; Jones and Cuello, l989). Using concurrent immunostaining for serotonin
(5-HT), Jones and Cuello (l989) revealed that after large WGA-HRP injections
into the globus pallidus/SI area, about 85% and 61% of retrogradely labeled
neurons were also 5-HT-positive in the DR and MR, respectively. On the other
hand, in the B9 cell group only 15% of the retrogradely labeled neurons
contained 5-HT. Interestingly, in the same study, the remaining neurons (l5%)
in the DR were positive for tyrosine hydroxylase (TH), indicating a
dopaminergic input to the area of globus pallidus/SI from this region. In
double-labeling experiments involving ChAT and PHA-L, we have found light
microscopic evidence suggesting input to BFC neurons from the DR (Fig. 10).
Confirmation of synaptic input remains to established at the electron microscopic
level.
There
have been several reports that MR stimulation produces hippocampal
desynchronization and inhibition of the bursting discharge of the septal
pacemaking cells (i.e. those neurons directly involved in controlling the
rhythmical slow-wave [theta] activity
of the hippocampus; Assaf and Miller, l978; Vertes, l981). MR-elicited desynchronization appears to be
a serotonin mediated effect (McNaughton et al., 1980). Although the MR can
influence the hippocampus directly, it is possible that it also may indirectly
influence it through GABAergic or cholinergic septohippocampal neurons, both of
which may be involved in pacing hippocampal theta
(Stewart and Fox, l990). However, there is no direct morphological evidence to
date for such connections.
DR
presumably serotonergic neurons show pacemaker-like activity and state-related
modulation of discharge (for ref. see Jacobs, 1987; Jacobs et al., l990;
Steriade and McCarley, l990). Phasic sensory (visual and auditory) stimulation
produces an excitation followed by
inhibition in these units
(Jacobs, l987). Injections of 5,7-DHT into brainstem serotonergic cell groups
produced a severe reduction of the
low-voltage fast activity in the neocortex that paralleled the extent of
serotonin depletion in the forebrain (Vanderwolf et al., l990). Moreover,
Vanderwolf and colleagues (Vanderwolf, l988; Vanderwolf et al.,l990) and
Riekkinen et al. (l990) have reported that combined cholinergic and
serotoninergic blockade in rat have potentiating effects on suppressing cortical arousal. Such animals also show severe memory
impairments (Nilsson et al., 1988; Richter-Levin and Segal, 1989). An
interaction between the serotonergic and cholinergic systems could take place
at the level of the target in the cortex and/or at the cholinergic cell body
level in the basal forebrain. Our preliminary morphological (Fig.10C) and
biochemical data (Záborszky and Luine, l987) are suggestive of a basal
forebrain interaction.
Reticular Formation
The
demonstration by Moruzzi and Magoun (l949) that the reticular formation (RF) is
involved in controlling the cortical EEG focused attention on the course and
termination of ascending reticular projections. After the initial Golgi and
degeneration studies (Nauta and Kuypers, l958; Scheibel and Scheibel, l958),
several subsequent papers used autoradiography (Edwards and de Olmos, l976;
Zemlan et al., l984; Eberhart et al., 1985; Jones and Yang, l985; Vertes et
al., l986; Vertes and Martin, l988) or retrograde techniques (Semba et al., l988;
Vertes, l988; Jones and Cuello, l989) to trace the ascending projections from
the brainstem RF. The reader should consult with the original papers or the
review by Vertes (l990a) for further details. Here we will summarize only data
relevant to basal forebrain projections.
In
general, long ascending reticular fibers travel through the central tegmentum
and are collected at the caudal diencephalon in Forel's fields. From this broad
band of fibers, three main fiber systems emanate: a dorsal one coursing into
the thalamus to innervate the intralaminar (parafascicular, paracentral,
central, lateral), midline (rhomboid, reuniens, paraventricular,
intermediodorsal), posterior, ventromedial, and dorsomedial nuclei; an
intermediate group of fibers, as the rostral continuation of Forel's fascicle,
passes through the subthalamus, giving off fibers to the zona incerta and
reticular thalamic nucleus, with some fibers reaching the basal ganglia; and a
ventrolateral pathway, which travels mainly within the medial forebrain bundle
(MFB) to innervate the basal forebrain. Although the different subnuclei of the
RF show overlapping projections, the more rostral the fibers originate, the
stronger the ascending projection. For example, the medullary parvicellular reticular
nucleus innervates mostly the lateral pontomedullary tegmentum, with only a few
fibers reaching the mesencephalic RF, and none reaching further rostrally. In
contrast, the nuclei gigantocellularis (Gc), pontis caudalis (RPc), pontis
oralis (RPo), and the midbrain reticular formation (MRF), give rise to
progressively heavier projections. Thus, only a few labeled fibers were
detected in the medial portion of the HDB and VDB from the Gc. In contrast, the
whole MS/VDB and medial two thirds of the ventral pallidum/HDB area are
innervated by the RPo. Finally, the MRF innervates the entire ventral pallidum,
globus pallidus, MS/VDB complex, parts of the caudate-putamen, the central
amygdaloid nucleus, lateral BSt, and medial prefrontal and suprarhinal cortices
(Jones and Yang, 1985).
Retrograde
studies suggest that ascending projections from more rostral portions of the RF
(e.g. MRF) project more dorsally in the basal forebrain, and may reach BFC
neurons in the SI/globus pallidus, while more caudally located RF cell groups
project mainly to the more ventral parts of the basal forebrain, such as the
HDB.
The
pontine RF appears to serve a prominent role in the generation of the
hippocampal theta rhythm. Petsche et
al. (1962) first suggested that discharges of a population of MS/VDB cells are
phase-locked with the hippocampal theta,
and showed further that these septal "pacemaking" cells were
activated by high frequency stimulation of the brainstem RF. Vertes (l977,
l979, 1990b) has identified, in the freely moving rat, a subset of RPo
neurons that fire selectively during
those states in which the theta
rhythm is present (waking, REM sleep). It is not clear, however, whether these
reticular axons directly contact cholinergic or GABAergic septohippocampal neurons,
or local interneurons in the septum.
On
the other hand, Steriade and colleagues (Steriade, l970; Steriade and Llinas,
l988; Steriade and McCarley, l990) proposed that MRF projections to the intralaminar thalamic nuclei may be
critically involved in the tonic activation processes related to EEG
desynchronization during waking and REM sleep. Although there is considerable
evidence for thalamic involvement, it is likely that the MRF projections to
areas rich in BFC neurons may be a route by which MRF acts upon the cortex
(Buzsaki et al., l988). PHA-L injections into the MRF result in massive
projections in the lateral part of SI (unpublished data), although the question
of whether these fibers contact BFC neurons awaits investigation at the EM
level. Brainstem activation of BFC might also come from the RPc, which has been
implicated in REM sleep (Greene et al., l989), although this possibility has
yet to be investigated.
Lateral Tegmental and Dorsal Medullary
Catecholaminergic Cell Groups
Intermingled
with other transmitter-containing neurons in the lateral and dorsal
medullary-pontine RF, several catecholaminergic cells were identified by
Dahlström and Fuxe (1964). The basal forebrain projections from these areas are
summarized below.
A7
area. The A7 noradrenergic cell group, which is located between the
ventrolateral border of the superior cerebellar peduncle and the lateral
lemniscus, constitutes a continuation of the A5 cell group. Although in combined
lesion-biochemical experiments it was reported that ascending noradrenergic
fibers from the area of the A7 cell group contribute to the innervation of the
hypothalamus (Palkovits et al., 1980), due to the fact that fibers originating
from more caudal noradrenergic cell groups project through the A7 area, these
results must be interpreted with caution. It is thus not clear at present
whether the A7 cell group projects to the basal forebrain.
A5
area. The A5 noradrenergic cell
group area (Dahlström and Fuxe, l964) is located in the caudal pons,
dorsolateral to the superior olive. The projections of the A5 noradrenergic
cell groups have been described recently by Byrum and Guyenet (l987).
Noradrenergic neurons from this region project to several hypothalamic,
thalamic, and limbic nuclei, and it is possible that a subpopulation of BFC
neurons in the caudal SI receive such input. In view of the widespread
interconnections of the A5 group with basal forebrain regions involved in
cardiovascular regulation (Guyenet and Byrum, l985), it is possible that this
information also reaches the BFC system.
A2
area. The A2 noradrenergic cell group is located in the caudal portion of
the nucleus of the solitary tract (NTS). It has been estimated that at least
90% of all nucleus commissuralis (caudal, noradrenergic part of the NTS)
neurons projecting through the MFB are catecholaminergic (Moore and Guyenet,
1983). Projections from this caudal part of the NTS in rat were followed to the
lateral PB area, SI, central amygdala and lateral BSt (Norgren, l978; Ricardo
and Koh, l978). It is thus possible that BFC cells receive general
viscerosensory input from the vagal nerve mediated through noradrenergic
afferents. On the other hand, different peptides such as enkephalin,
somatostatin, or substance P have been localized in ascending projections from
the caudal NTS (Riche et al., l990; Sawchenko et al., 1990), and are known to
be present in fibers within forebrain regions containing BFC neurons.
Therefore, it is possible that BFC neurons receive some peptidergic projections
from the NTS.
Several
studies have shown that serotonin applied directly to the area of the NTS and immediate surrounding region of the
lower medulla elicits cortical synchronization and/or sleep. It has further
been proposed that serotonin afferents to the NTS responsible for triggering
slow wawe sleep may primarily arise from the area postrema (for ref. see
Vertes, l990b). It is unclear whether
information relayed to the cortex from this "medullary sleep center"
is mediated via BFC or other basal
forebrain neurons.
A1
area. The A1 noradrenergic cell
group area (Dahlström and Fuxe, 1964) is located caudally within the
ventrolateral medulla. The projections from the A1 area differ significantly
from those of each of the reticular nuclei discussed above (McKellar and Loewy,
l982; Vertes et al., l986). Although some fibers project to the midline
thalamus, zona incerta and rostral intralaminar thalamic nuclei, a considerable
proportion of fibers ascend in the medial forebrain bundle (MFB) and terminate
in various hypothalamic nuclei, as well as in areas containing BFC neurons such
as the SI, and MS/VDB complex. Using PHA-L tracing from a region which included
the A1 catecholaminergic cell group (Fig. 11), labeled fibers could be traced
to the vicinity of BFC neurons in the medial HDB and ventral part of the globus
pallidus, although confirmation of synaptic contacts awaits ultrastructural
examination.
GABA
GABAergic
synapses on cholinergic projection neurons were first described in the ventral
pallidum (Záborszky et al., 1986b), and have since been identified on
corticopetal, presumably cholinergic neurons in the globus pallidus (Ingham et
al., l988), on septal cholinergic neurons (Leranth and Frotscher, l989), and on
cholinergic cells in the anterior amygdaloid area (Nitecka and Frotscher,
l989). GABAergic boutons form symmetric membrane specializations (Fig. 14D),
which in some areas comprise over half of the input to the perikarya and
proximal dendrites (Ingham et al., l988). In the ventral pallidum, a
significant proportion of GABAergic terminals comes from the nucleus accumbens,
since lesions of this structure result in a significant decrease of GAD in the
ventral pallidum (Walaas and Fonnum, l979; Záborszky et al., l982). Another
source of GABAergic terminals may be local GABAergic neurons, such as
postulated for the septum (Leranth and Frotscher, l989).
There
is ample evidence that the turnover rate of hippocampal acetylcholine (ACh), as
well as theta activity, can be
manipulated by intraseptal administration of GABAergic agents (Wood and Cheney,
1979; Costa et al., l983; Allen and Crawford, l984; Blaker et al., l986).
Changes in cortical acetylcholine turnover rate (AChTR), sodium
dependent high affinity choline uptake (SDHACU), or cortical ACh output, have
also been reported following muscimol injections into the SI (Wood and Richard,
l982; Wenk, l984; Blaker, l985; Casamenti et al., 1986; Wood and McQuade,
l986). The pharmacological studies have indicated further that muscimol acts
through GABA-A receptors located on the cholinergic neurons (Blaker et al.,
l986). It has been also suggested that other septal neurotransmitters such as
dopamine, glutamate, and ß-endorphin modulate AChTR indirectly
through their influences on GABAergic interneurons (Costa et al. 1983). Whether
the GABAergic link indeed acts as a "final common pathway" (Chrobak
et al., l989) for these afferents in the regulation of septohippocampal neurons
is unresolved, since it is unknown whether GABAergic interneurons which receive
such afferents project, in turn, to septohippocampal or corticopetal
cholinergic neurons.
GABAergic
input to BFC neurons may have functional significance with respect to learning
and memory processes, as pharmacological manipulation of GABA-A receptors
within the medial septum or nucleus basalis has been shown to affect
performance on working memory tasks, as well as in inhibitory avoidance
behavior (Nagel and Houston, 1988; Chrobak et al., 1989). Also, enhancement of
cholinergic function through pharmacological disinhibition of the
GABAergic-cholinergic interaction has been suggested as a therapy for dementia
of the Alzheimer's type (Sarter et al., 1988, 1990).
Acetylcholine
It
has been generally noted that cholinergic neurons do not receive significant
cholinergic input (Ingham et al., l985; Armstrong, 1986; Dinopoulos et al.,
l986; Záborszky et al., 1986b; Bialowas and Frotscher, l987; Martinez-Murillo
et al., 1990). We have occasionally
found ChAT-immunoreactive boutons in contact with cholinergic cell bodies or
proximal dendrites in the SI, and these synapses were usually of the symmetric
type (Fig. 14). Since symmetric type synapses have generally been associated
with inhibitory synaptic effects (Peters et al., l976), this finding is not
readily compatible with electrophysiological studies which have indicated that
the majority of septohippocampal or corticopetal neurons are excited by the
iontophoretic application of ACh
(Lamour et al., l984a; 1986). These effects might be explained by an
action through inhibitory interneurons, however. Also, physiological studies
have indicated that the action of ACh within the CNS is quite diverse, and may
largely depend upon the nature of the postsynaptic receptor (Nicoll,
l988).
The
origin of cholinergic axons in the basal forebrain remains unclear. One
possible source is the ascending cholinergic projection from the PPT/LDT (Satoh
and Fibiger, l986), although a PHA-L study did not find evidence for
terminations on BFC neurons, but rather, on non-cholinergic cells (Hallanger et
al., 1988). In another study, physiologically identified corticopetal neurons
in the SI were observed to have local axon collaterals (Semba et al., l987),
however, since the chemical nature of these neurons was not confirmed it is
unclear whether they were indeed cholinergic.
Glutamate
It
has been suggested that amygdalofugal axons use glutamate as transmitter,
as evidenced by decreases in glutamate uptake in the SI following amygdaloid
lesions (Francis et al., l987), as well as by the presence of 3H-D-Asp
containing cell bodies in the amygdala after injection of this tracer into the
ventral pallidum (Fuller et al., l987). We have found that asymmetric contacts
are formed on BFC neurons by amygdalofugal axons (Záborszky et al., 1984a),
although the transmitter of these terminals was not identified. In addition, hippocampal
efferents to the lateral septum apparently use also glutamate as a transmitter,
as evidenced by decreases in glutamate uptake after surgical lesions of the
fimbria (Fonnum and Walaas, 1978). Injections of glutamate in the SI
significantly increased cortical SDHACU (Wenk, 1984), suggesting that corticofugal
axons are also likely to be glutamatergic. A recent retrograde tracing study
injecting 3H-D-Asp into
different basal forebrain areas containing BFC neurons suggests a more
widespread origin of glutamatergic projection to basal forebrain areas,
including the intralaminar thalamic nuclei, lateral septum, habenula, and
several hypothalamic and brainstem sites (Carnes et al., l990). Glutamic acid
has been shown to cause rapid depolarization of cultured cells from the nucleus
basalis (Nakajima et al., 1985), although the identification of glutamate in
synapses on BFC neurons awaits ultrastructural double-labeling studies.
Substance
P-containing terminals have been found to contact BFC neurons in the ventral
pallidum and ventromedial globus pallidus (Bolam et al., 1986). Such synapses
were detected on the proximal dendrites and cell bodies of cholinergic neurons.
It has been estimated that as many as one-third of the observed contacts
contained substance P. The majority of immunoreactive terminals formed
symmetrical synaptic specializations. However, a few boutons formed asymmetric
contacts with the proximal dendrites of cholin-ergic neurons. Individual
cholinergic neurons apparently receive multiple substance P-containing
terminals. A light microscopic study performed on human tissue has suggested
that substance P innervation of acetylcholine esterase (AChE)-positive nucleus
basalis neurons is heterogeneous, since in some areas all AChE-positive cell
bodies were in possible contact with approximately 5-20 substance
P-immunoreactive terminal-like structures, while in other areas AChE positive
neurons were entirely devoid of such putative contacts (Beach et al., 1987).
One potential site of origin of substance P-containing terminals to BFC neurons
in the ventral pallidum or ventromedial globus pallidus is the nucleus
accumbens, since lesions of this region cause a reduction of substance P
immunostaining in the ventral pallidum (Záborszky et al., 1982; Haber and
Nauta, l983). However, the different morphological types of substance P
synapses, as well as the fact that several other structures which project to
the areas containing BFC neurons also contain substance P-positive cells,
suggests other potential input sources, including the amygdala, BSt,
hypothalamus, and the pontine tegmentum (see Beach et al., l987).
Iontophoretic
application of substance P has been shown to produce increased excitability of
cholinergic neurons from cultures of the medial septum and diagonal band, an
effect caused by inhibition of inward rectifying K-channels (Nakajima et al.,
1985, l988). Moreover, intraseptal application of substance P facilitates
performance in passive avoidance behavior (Staubli and Huston, l980). These
findings are in apparent contradiction to the effects of intraseptal
administration of substance P on hippocampal AChTR, which has been
found to be inhibitory (Malthe-Sorensen et al., l978a). Studies on the effects
of muscimol and substance P on the AChTR in different regions of the
hippocampus suggest that these two substances control two different projections
from the septum to the hippocampus (Blaker et al., l984). Namely, septal
administration of substance P in the MS/VDB resulted in reduction of AChTR
only in the dorsal hippocampus, while muscimol affected only AChTR
in the ventral hippocampus. Considering the topographical arrangement of
cholinergic neurons projecting to the dorsal versus the ventral hippocampus
(Amaral and Kurz, l985), it is possible that distinct populations of
cholinergic neurons in the septum receive GABA or substance P input. At present,
however, direct morphological data are lacking for such a possibility. Similar
to septal injections, administration of substance P into the region of the
nucleus basalis in rat facilitates performance of an inhibitory avoidance task
(Kafetzopoulos et al., 1986; Nagel and Huston, 1988).
Enkephalin
An
immunocytochemical double-labeling experiment has shown that
enkephalin-positive terminals contact with cholinergic dendrites in the globus
pallidus (Chang et al., l987), although these were reported to be few. Another
study (Martinez-Murillo et al., l988a) found somewhat more enkephalin-positive
terminals in contact with AChE-positive neurons in the globus pallidus and
internal capsule. Although technical factors can limit the detection of
enkephalinergic/cholinergic interactions, these studies appear to suggest that
there is little monosynaptic interaction between enkephalinergic terminals and
cholinergic neurons.
Injections
of enkephalin or its derivatives to the SI have been shown to reduce neocortical
SDHACU by approximately 50% (Wenk, l984), and produce naloxone-reversible
locomotor hyperactivity (Baud et al., l988). In another study, however, local
delivery of enkephalin derivatives into the SI failed to induce changes in
cortical AChTR, although a significant decrease resulted after
parenteral administration (Wood and McQuade, l986). On the other hand, septal
injections of enkephalin have been found to cause a significant decrease in
hippocampal AChTR (Costa et al., l983). Clearly, the role of enkephalin
in modulating cortical or hippocampal cholinergic activity requires
clarification in further anatomical and pharmacological studies.
Somatostatin
has been found in axon terminals in contact with BFC neurons in the SI
(Záborszky, l989a). The synapses observed were of the symmetric type, and were
found primarily on proximal dendrites (Fig. 14B). The results of a high
magnification light microscopic analysis of the distribution of putative
contact sites suggest that BFC neurons in the SI and MCP/HDB may receive a
rather diffuse innervation (Záborszky et al., in preparation). At least a
proportion of such terminals are likely to originate from local interneurons.
Cysteamine-induced
release of somatostatin has been shown to lead to significant impairment of
cognitive processes such as on retention in passive avoidance tasks (Vecsei et
al., 1984; Haroutunian et al., 1987). Since combinations of nucleus basalis
lesions and cysteamine depletion in rats did not lead to any greater impairment
of mnemonic function than that produced by ablation of the nucleus basalis
alone, it was suggested that the amnesia produced by the cysteamine-induced
release of somatostatin is mediated through the BFC system (Haroutunian et al.,
l989). Our morphological demonstrations of symmetric synapses on cholinergic
corticopetal neurons suggests that somatostatin released at these synapses may
inhibit cholinergic function in target areas. Somatostatin has been reported to
inhibit ACh release from cholinergic neurons of the myenteric plexus (Yau et
al., l983), although it has been shown that intraventricular injection of
somatostatin increases hippocampal AchTR (Wood et al., l979). The
functional impact of such somatostatinergic/cholinergic interactions in the basal
forebrain remains to be elucidated.
Neuropeptide
Y (NPY) has been found in axon terminals in synaptic contact with BFC neurons
in the SI (Záborszky and Braun, l988). In many cases, multiple NPY-containing
boutons were found to encompass cholinergic cell bodies. The synapses were
always of the symmetric type. Similar to somatostatin, at least a proportion of
NPY terminals are likely to be derived from local interneurons. Light
microscopic observations have suggested that a single peptidergic neuron (NPY
or somatostatin), with its locally arborizing axon collaterals, may innervate a
number of BFC neurons. Conversely, a single BFC neuron may receive axon
terminals from several NPY or somatostatin cells (Záborszky, 1989b).
It
has recently been demonstrated that intraventricular NPY administration
modulates retention of foot-shock avoidance in a dose and time-dependent manner
(Flood et al., l987). Post-training administration of NPY to the septum or
hippocampus also has been demonstrated to affect performance on a learning task
(Flood et al., 1989). Whether these effects are mediated in part through the
BFC system is presently unknown. Clearly, detailed anatomical and
pharmacological studies are necessary to reveal the possible significance of
NPY-cholinergic interactions in the basal forebrain.
Other Peptides
Various
peptide-containing fiber systems are differentially distributed in the basal
forebrain (for ref. see Palkovits, l984). As a result, different populations of
BFC neurons are likely to be contacted by different afferent peptidergic fibers
(Záborszky, l989b). For example, the majority of BFC neurons in the rostral
globus pallidus and ventral pallidum may receive a rich neurotensin innervation
(see Fig. 12), but appear to be contacted only occasionally by other
peptidergic afferents. BFC neurons in the SI appear to receive a substantial
input from a number of different peptidergic systems, including NPY, somatostatin and neurotensin.
The
possibility of a rich innervation of a subpopulation of BFC neurons by neurotensin
fibers is supported by a study reporting localization of radiolabeled
neurotensin binding sites on AChE-positive neurons in the area of the nucleus
basalis (Szigethy et al. l990). Since intraventricular administration of
neurotensin in the rat has been shown to modulate hippocampal AChTR
(Malthe-Sorenssen et al., l978b), as well as to affect performance in a
conditioned avoidance paradigm (van Wimersma Greidanus et al., l982), it is
possible that neurotensin modulation of BFC output may have a role in memory
processes.
Vasopressin
axons emanating from the hypothalamic paraventricular nucleus en route to the posterior pituitary cross
cholinergic dendrites of the SI or HDB, and boutons containing large
neurosecretory vesicles are occasionally found in synaptic contact with distal
cholinergic dendrites (unpublished data, Fig. 14C). The functional significance
of such connections is presently unclear.
Galanin
has been shown to be present in neurons in the area of the basal nucleus of
Meynert in the normal human brain, both in local circuit neurons, and in a
number of cholinergic projection neurons. In addition, local galanin
interneurons appear to innervate cholinergic cells (Chan-Palay, l988). Lesion studies
suggest that most medial septal neurons that contain both galanin and
acetylcholine project to the ventral hippocampus (Melander et al., l986). In
the ventral hippocampus exogenously administered galanin appears to attenuate
scopolamine-stimulated ACh release (Fisone et al., l987), and inhibit the
muscarinic stimulation of phosphoinositide turnover (Palazzi et al., l988).
Galanin also has been shown as an
inhibitory peptide affecting myenteric cholinergic neurons (Yau et al., l986),
although no data are yet available for their central action at the level of the
cholinergic neurons in the basal forebrain. In Parkinson's disease and
Alzheimer's disease, there is an apparent hyperinnervation of surviving
cholinergic neurons, and it has been proposed that this hyperinnervation
reflects plasticity in response to altered (diminished) cholinergic function.
However, since galanin might act as an inhibitory transmitter, the effect might
be a further deterioration of the cholinergic metabolism (Chan-Palay, l988).
Behavioral studies in rats suggest that galanin acts as an inhibitory modulator
of ACh, having no direct effect of its own, but partially blocking the
facilitatory actions of ACh on working memory (see Crawley and Wenk, 1989).
In
addition to galanin, studies have indicated changes in levels of somatostatin,
neurotensin, NPY and a-melanocyte-stimulating-hormone in the basal forebrain
areas rich in cholinergic neurons in Alzheimer's disease (Ferrier et al.,
1983; Allen et al., l984; Arai et al.,
l986; Constantinidis et al., 1988). It is interesting to note that in
Alzheimer's disease, individual sectors of the nucleus basalis are affected
differentially, with concomitant pathological changes in the corresponding
cortical projection areas (Arendt et al., l985). Our findings (Záborszky,
l989b) may be relevant in this context, in that specific populations of
cholinergic neurons are likely to be contacted and modulated by specific sets
of peptidergic afferents. It will therefore be important to determine
whether altered peptidergic innervation
in the basal forebrain is correlated with the pathological changes in subsets
of nucleus basalis neurons in Alzheimer's disease.
Catecholamines
Noradrenaline/Adrenaline. We have recently mapped the distribution of
dopamine-ß-hydroxylase (DBH) positive varicosities in relation to BFC system at
the light microscopic level. With the exception of those neurons in the dorsal
part of the globus pallidus and internal capsule, cholinergic neurons are
approximated by DBH-positive varicosities in most portions of the BFC system.
In some cases, particularly within the caudal SI, distal segments of
cholinergic dendrites appeared to receive repetitive contacts in the form of a
"climbing" arrangement. The
distribution of DBH-positive fibers/terminals in relation to BFC neurons at the
level of the anterior commissure is shown in Fig. 13A. Parallel experiments at
the EM level confirmed synaptic contact between DBH-positive terminals and
cholinergic neurons (Záborszky et al., l991). Fig. 13B illustrates the
distribution of PHA-L labeled fibers in relation to BFC neurons following PHA-L
injection in the locus coeruleus, suggesting that at least part of the noradrenaline innervation of the
basal forebrain originates in the locus coeruleus. The results of these studies
have suggested that the overall noradrenergic innervation of the BFC system is
rather diffuse (see Fig. 15C), particularly from the locus coeruleus (Fig.
15B), although the input is apparently not entirely uniform. Regional
variations in the type and distribution of labeled fibers further suggested
contributions to the innervation of the BFC from noradrenergic cell groups such
as the A1 and A2 groups.
Parenteral
administration of amphetamine increases AChTR in the hippocampus
(Costa et al., l983). This response is likely to be mediated in part through
noradrenergic terminals on septal BFC cells, since the response is prevented by
intraseptal injection of phenoxybenzamine, an irreversible a-adrenergic blocker
(Costa et al., l983), or by 6-OHDA lesions of the ventral or dorsal
noradrenergic bundles (Robinson, l986, l989). Furthermore, it is suggested that
this noradrenergic input phasically activates the septohippocampal cholinergic
neurons during working memory testing (Durkin, l989). Other pharmacological
experiments also have implicated noradrenergic-cholinergic interactions in
memory related processes (Mason and Fibiger, l979; Decker and Gallagher, l987;
Decker and McGaugh, l989; Decker et al., l990; Haroutunian et al., l990),
although the site(s) of such interactions were not determined. The present
findings might provide a morphological basis for such effects.
Dopamine. Biochemical, pharmacological,
electrophysiological, histofluorescence, and combined retrograde immunohistochemical/tracing
studies have suggested dopaminergic innervation of forebrain regions containing
BFC neurons (Brownstein et al., 1974; Versteeg et al., l976; Lindvall and
Björklund, l979; Haring and Wang, l986; Martinez-Murillo et al., l988b; Semba
et al., l988; Jones and Cuello, l989;
Napier and Potter, l989; Napier et al., this volume). We recently mapped the
distribution of TH-positive axonal varicosities in relation to cholinergic
neurons (Záborszky et al., l991). Due to the fact that the TH antibody used in
our study labeled many fibers in the basal forebrain that were also
DBH-positive, interpretation of these data requires caution. However, in areas
such as the ventromedial globus pallidus and the internal capsule, where few or
no DBH-positive terminals were present, it is likely that TH varicosities
represent dopaminergic terminals, and preliminary EM evidence indicates that TH
axons in these regions establish symmetric synapses with BFC cells.
Pharmacological
studies suggested that septohippocampal BFC neurons are under a tonic
inhibitory dopminergic influence from the ventral tegmental area, and that this
inhibitory effect of dopamine on hippocampal AChTR is mediated through the septum via GABAergic
interneurons (Robinson et al., l979; Costa et al., l983; Gilad et al., 1986).
An indirect dopaminergic influence on the corticopetal cholinergic neurons has
also been suggested (Casamenti et al., l986). Several recent pharmacological
studies have suggested that dopaminergic/cholinergic interactions play some
role in cognitive functions, including spatial memory performance (Galey et
al., l985; McGurk et al., l988; l989; Levin et al., l990). A definitive answer
to the question of direct dopaminergic/cholinergic interactions in the basal
forebrain awaits double-labeling studies using antisera for ChAT and dopamine.
In addition, further morphological and biochemical studies may reveal the
significance of a possible GABAergic mechanism mediating this interaction.
Studies
on aging and age related disorders such as Alzheimer's disease and
Parkinson's disease suggest that the disruption of catecholaminergic/
cholinergic interaction in these conditions may contribute to the cognitive
decline observed. In aged rodents, nonhuman primates, and humans, reductions in
forebrain catecholaminergic markers have been reported (Carlsson, l987; Morgan
et al., l987), as has cell loss within the substantia nigra and locus coeruleus
(Brody, l976; McGeer et al., 1977; Chan-Palay and Asan, l989a). Forebrain
catecholaminergic markers are also reduced in Alzheimer's disease (for
ref. see Mann, l988) and neuronal loss has been reported within the locus
coeruleus and ventral tegmental area (Forno, l978; Tomlinson et al., 1981;
Ichimaya et al., l986; Price et al., l986; Palmer et al., l987; Chan-Palay and
Asan, l989b). In Parkinson's disease, in addition to the well known
degeneration of the substantia nigra, neuronal loss within the locus coeruleus
and reductions of forebrain noradrenaline levels have been demonstrated (Mann
and Yates, 1983; Chan-Palay and Asan, 1989b). Reductions in forebrain
presynaptic cholinergic markers and cell loss in the nucleus basalis have been
reported not only in Alzheimer's diseases but also in aging, as well as in
Parkinson's disease (McGeer et al.,
l984; Decker, l987; Fisher et al., l989; Jellinger, l990). The extent to which
reductions in catecholaminergic and cholinergic markers are independent or
related processes is unclear. However, the fact that 6-OHDA lesions of the
ascending catecholaminergic bundle result in a decrease in ChAT activity in
forebrain areas rich in cholinergic cell bodies (Záborszky and Luine, l987;
Záborszky et al., l991) suggests a catecholaminergic influence on BFC neurons.
The lack of this influence could be a factor in the metabolic deterioration of
the BFC in Alzheimer's disease,
Parkinson's disease, or aging, a notion consistent with a recently proposed
theory of transynaptic systems degeneneration in neurological diseases (Saper
et al., l987).
Recent
attempts to assess inputs to BFC neurons have employed double-labeling
approaches involving ChAT immunocytochemistry combined with either tracing or
transmitter identification at the electron microscope level (Záborszky et al.,
l984a; Ingham et al., l985; Bolam et al., 1986; Záborszky et al., 1986b; Chang
et al., 1987; Ingham et al., 1988; Martinez-Murillo, et al., 1988a; Záborszky
and Cullinan, 1989; Záborszky et al., 1991). Fig. 14 shows several examples of
synapses on identified cholinergic neurons. As suggested in the schematic
drawing of Fig. 14A, various inhibitory synapses appear to show a preferential
distribution on the cell body or proximal part of the dendrite. These terminals
are likely to originate mainly from local interneurons. On the other hand,
excitatory inputs from more distant areas apparently concentrate on more distal
dendritic segments. This afferent "synaptic topography" (Smith and
Bolam, l990) based on the available data may represent a first level of
integration of afferent information. However, since the number of synapses
and/or their types may vary according
to the location and/or the target of the cholinergic neuron, the minimum operational
network for corticopetal cholinergic neurons remains to be established. Another
level of interaction between inputs may be represented through local GABAergic
and peptidergic interneurons (Záborszky, l989a,b), which project to cholinergic
neurons. Although the exact divergence-convergence relationships of these
interneurons, as well as their afferents are unknown, it is likely that such an
interactive system signifies complex processing of information through the cholinergic system prior to its
reaching the cortex.
For
practical purposes only a limited number of neurons with their afferent inputs
can be reconstructed at the ultrastructual level. To assess the organization of
different afferent systems in relation to the cholinergic projection system in
its entirety, we mapped the potential
sites of contact under high resolution light microscopy (e.g. Fig. 15).
Although this technique is subject to several limitations (see Cullinan and
Záborszky, 1991), it does appear to provide insight into the pattern of
innervation from a given brain region (see Fig. 15).
On
the basis of data using this "double strategy" of identifying
terminals on single cells, as well as mapping their quasi 3-D distribution, (Záborszky and Cullinan, l989; Cullinan and Záborszky, l991; Záborszky et
al., l991), a number of organizational principles have emerged that are likely
to be relevant not only to the cases studied, but with respect to afferents to
the BFC system in general: 1) Inputs to the BFC system are apparently non-specific. In all cases
examined, labeled terminal varicosities detected in the basal forebrain were
related to both cholinergic and non-cholinergic elements. Indeed, the vast
majority appeared to be associated with elements that were non-cholinergic.
These observations support the notion that cholinergic neurons do not maintain
afferent connections distinct from neighboring non-cholinergic cells, but
rather, participate to some extent in the circuitry of the forebrain regions in
which they are located, as has been suggested by Grove et al., (l986). 2)
The distribution patterns of various terminals on the BFC sytem correspond to
the general topographical arrangement of basal forebrain fibers. For example,
fiber contingents coursing through certain compartments of the MFB tend to have
terminations in the rostral forebrain corresponding to the same compartment.
Moreover, projections in which fibers ascend through multiple MFB compartments
tend to innervate the forebrain in a more diffuse fashion. A precise
localization of different ascending brainstem fiber systems in relation to the
compartments of the MFB (see Satoh and Fibiger, 1986) may therefore be of value
in defining inputs to chemically specific cells in the basal forebrain,
including BFC neurons. 3) Afferents to the BFC system may be restricted or
relatively diffuse. The majority of afferents examined in our experiments
showed a preferential distribution towards subsets of BFC neurons. For example,
inputs from paralimbic cortical areas were confined to subterritories of the
basal forebrain, and similarly, those from the hypothalamus or the ventral
striatum reached subpopulations of these neurons. In addition, the distribution
of several peptides and other afferents (see Semba and Fibiger, l989;
Záborszky, l989b) to the basal forebrain suggest that they might contact
subpopulations of BFC cells. Thus, the emerging view is that different subsets
of these cells receive different combinations of afferents according to their
location in the basal forebrain. On the
other hand, noradrenergic afferents, particularly those from the locus
coeruleus, apparently contact extended
portions of the BFC system. Other afferents, particularly those which comprise
"diffuse corticopetal systems" (Saper, 1987), such as the raphe
nuclei, might similarly be expected to maintain a diffuse or generalized
relationship to the BFC system.
It has been suggested by several authors that
BFC neurons receive afferents from those fiber systems with which they are
associated in the basal forebrain. Recent morphological data obtained with the
PHA-L technique, however, have underscored the distinction between terminal
fields and fibers of passage in these areas (Grove, l988; Cullinan and
Záborszky, l991). For example, in some cases, fibers completely devoid of
terminal varicosities were noted to course through areas rich in BFC cells. On
the other hand, the detectability of afferents to cholinergic neurons was
usually proportional to the density of terminals present in a given area.
Clearly, a more complete understanding of the afferent connections of the BFC
system will require the disclosure of those sources which elaborate significant
terminal networks in the forebrain areas containing these neurons.
BEHAVIORAL AFFILIATIONS OF BFC NEURONS AS RELATED TO SPECIFIC CIRCUITS
Various
authors have emphasized the role of the BFC system in arousal, sensory
processing, emotion, motivation, learning, memory and motor functions. These
processes may occur serially, even if they are often interactive. Much connectional information is needed to
establish the possible role of the BFC in these functions, although as
discussed below, these processes may be mediated by distinct neural circuits
involving BFC neurons.
Moruzzi
and Magoun (l949) demonstrated that electrical stimulation of the midbrain
reticular formation (MRF) evokes a general cortical EEG desynchronization
similar to natural arousal. Since no direct connections exist between the MRF
and the cortex, the facilitatory effect of MRF stimulation on the cortex was
thought to be mediated partially through the thalamus and through an
ill-defined extrathalamic route (Lindsley et al., l949; Starzl et al., 1951).
Although there is considerable evidence for the involvement of the intralaminar
thalamic nuclei on cortical arousal
(see reviews by Steriade, 1970; Steriade and Llinas, 1988; Steriade and
McCarley, 1990), a growing number of studies have suggested that at least part
of the effect of MRF stimulation on cortical or hippocampal activation is
mediated through the BFC system (Dudar, 1977; Buzsaki et al., l988; Semba, this
volume).
Several
data have been taken in support of a role of the BFC in cortical activation. 1)
Cortical release of ACh is closely related to level of EEG arousal (Kanai and
Szerb, l965; Celesia and Jasper, l966; Collier and Mitchell, l967; Szerb, l967;
Jasper and Tessier, l971). 2) Data correlating behavioral states with
EEG-related discharge profiles of nucleus basalis neurons (Pirch et al., 1986;
Detari and Vanderwolf, 1987; Buzsaki et al., l988; Szymusiak and McGinty,
l989), together with electrophysiological evidence that ACh acts as a slow
excitatory neurotransmitter in the neocortex (Sillito and Kemp, l983), have
been taken as support for the hypothesis that the nucleus basalis provides a
steady background of neocortical activity that may enhance the effects of other
afferents to the neocortex. 3) Lesions
of the basal forebrain lead to decreases in ACh activity in the cortex and
reductions of electrical activity over the lesioned hemisphere (LoConte et al.,
l982). 4) EEG alterations with an increased tendency toward slow waves are seen
in patients suffering from Alzheimer's disease (Coben et al., l983), in which
loss of neurons in the nucleus basalis is reliably observed.
An
important issue is thus what drives the
BFC system, particularly since these neurons do not show pacemaker-like
activity (Griffith, 1986, l988, this volume).
The question of how messages from the outside world (during the waking
state) or from the millieu intérieur (as during REM sleep) drive the BFC system
remains largely unanswered. Since MRF stimulation evidently involves not only
activation of cells, but of several passing fiber systems, various potential
sources of afferents might be considered based upon the correlation between
their discharge patterns and the EEG. For example, locus coeruleus axons appear
to contact extended portions of the BFC system, and physiological studies have
implicated the locus coeruleus in cortical arousal. Although direct projections
from various other brainstem sites to BFC neurons are possible, the effects of
MRF stimulation upon the cortex could be mediated in part through the lateral
hypothalamus, which is known to receive brainstem afferents, as well as contact
BFC neurons.
Sensory
information passes through multi-synaptic pathways from primary sensory
cortical areas, through multi-modal association areas, to the amygdala and
hippocampus (Jones and Powell, l970; van Hoesen and Pandya, 1975; Turner et
al., l980; Pandya and Yeterian, 1985; Amaral, l987; Heilman et al., l987). It
has been suggested that cortico-amygdaloid connections are particularly
important in the affective processing of sensory signals (see LeDoux, l987) and
in stimulus-reinforcement associations (Jones and Mishkin, l972). The results
of biochemical measurements of ChAT activity in various cortical regions
suggested that sensory information is likely to come under progressively
greater cholinergic influence as it is transferred from sensory association
areas, to the multimodal association areas, and finally to the amygdala
(Mesulam et al., 1986b). In other words, BFC neurons may gate cortico-limbic
interactions. More specifically, Rolls and colleagues (Rolls, l989; Wilson and
Rolls, l990a,b) proposed that cholinergic neurons from the orbitofrontal cortex
and the amygdala receive information on the expected availability of
reinforcement brought about through learning, and relay this information to
widespread cortical areas to facilitate sensory, motor, or associative
functions. Indeed, neuronal responsiveness in sensory cortex appears to be
related to similar motivational changes in basal forebrain neurons (Steriade
and McCarley, l990). It is unclear, however, how these motivational related
events are transmitted to widespread cortical areas, since it appears that
those BFC neurons that receive orbitofrontal input project to limbic cortical
areas, rather than sensory or motor cortical regions.
Studies
involving pairing of sensory
stimulation with cortical application of ACh suggest that ACh can produce
stimulus-specific modification of information processing in sensory cortical
areas (Ashe et al., l989; McKenna et al., l989; Metherate and Weinberger,
1990). Furthermore, studies using
sensory stimulation in combination with nucleus basalis lesions (Satoh
et al., 1987; Ma et al.,l989; Juliano et al.,l990) or basal forebrain
stimulation (Pirch et al., l986; Rigdon and Pirch, 1986; Rasmusson and Dykes,
1988; Pirch et al., this volume), suggest that the enhancement of
sensory-related cortical responses is correlated with activation of the BFC
system. Although more elaborate
functional studies are necessary, the connectional data reviewed here are
consistent with the notion that sensory
stimuli may reach specific cortical areas through relatively localized portions
of the BFC system. For example, as discussed in the section on brainstem
connections, auditory signals with weak tuning properties may reach a
subpopulation of cholinergic neurons through the peripeduncular area. Such
cholinergic neurons then would project to the auditory cortex (see Fig. 9A). A
similar situation may exist for viscerosensory inputs as discussed and
summarized in Fig. 9B. In this way, specific sensory cortical areas might
receive a relatively primitive representation of the peripheral stimulus
through the cholinergic system, and more precise information through the
thalamic relay. This mechanism might allow for the release of ACh that is
spatially and temporally coupled with the arrival of specific sensory signal
through the thalamic relay nuclei.
A
similar coupling of ACh release with increased cell firing in the motor cortex
may form the basis for learned motor performance (Woody, 1982; Richardson et al., l988; Richardson and DeLong,
l991), although the connectional basis for this is more uncertain.
Recent
anatomical studies has suggested that afferents to BFC neurons may contact
widespread portions of this system, or be relatively restricted. Generalized
behavioral functions such as arousal may be mediated in part through relatively
diffuse inputs, such as the noradrenergic afferents from the locus coeruleus.
Restricted afferents may be related to more specific functions. However,
generalized versus specific functions of this system may not be mutually
exclusive alternatives, in that either mechanism might predominate depending
upon the current or prevailing state of afferent control.
It
is also important to note that afferents to BFC neurons are non-specific, since
neighboring non-cholinergic neurons are also apparently the recipients of a
given afferent projection. These non-cholinergic cells may have efferent
projections that parallel those of BFC neurons, or may constitute interneuronal
populations, which in turn, innervate BFC cells. Thus, it is conceivable that
such arrangements represent local integrative processing units related to
specific functions, of which cholinergic neurons comprise a part.
Thus,
future anatomical studies should be directed at distinguishing subpopulations
of BFC neurons with their input-output relationships and to define to what
extent such cholinergic neurons together with other nearby non-cholinergic
neurons participate in specific forebrain circuits. Such detailed anatomical
data, together with the information outlined above and from other recent
functional studies (e.g. Olton et al., l991; Richardson and DeLong, l991),
should aid in the design of pharmacological, electrophysiological, and
behavioral investigations of the functional roles of specific basal forebrain
circuits.
Particular
thanks are due to Dr. Lennart Heimer, who gave continuous support and
encouragement over many years. Mr. F.
Lee Snavely and Ms. Vinessa Alones have provided skillful assistance. The
original research summarized in this review is supported by USPHS Grants Nos.
23945 and 17743.
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Fig. 1. A-F: Series of drawings made from
coronal sections through a rat brain (rostral to caudal) that have been immunostained
for ChAT (choline acetyltransferase), illustrating the distribution of
cholinergic neurons (dots). Striatal cholinergic neurons (including those in
the ventral striatum) have been omitted for simplicity. Abbrev. ac =
anterior commissure; Acb = accumbens nucleus; BL = basolateral amygdaloid
nucleus; BSt = bed nucleus of the stria
terminalis; CA = central amygdaloid nucleus; CP = caudate putamen; DM =
dorsomedial hypothalamic nucleus; f = fornix; GP = globus pallidus; HDB = horizontal limb of the diagonal band;
ic = internal capsule; LSd = lateral septal nucleus, dorsal; LSi = lateral
septal nucleus, intermediate; LV =
lateral ven-tricle; MCP = magnocellular preoptic nucleus; MS = medial septal
nucleus; ot = optic tract; Rt = reticular thalamic nucleus; SI = sublenticular
substantia innominata; sm = stria medullaris; VDB = vertical limb diagonal band
nucleus; VM = ventromedial hypothalalamic nucleus; VP = ventral pallidum.
Fig. 2. Composite map illustrating the distribution of
cholinergic projection neurons (dots). This drawing was composed from 6 camera
lucida drawings which were aligned and superimposed to generate the final
figure. Only the ventral outlines of the brain and the corpus callosum are
indicated. The same technique is used to show the distribution of putative
contact sites of different afferent systems in relation to the cholinergic
neurons in Fig. 15. Cells between the arrows correspond to the area of the SI. Abbrev.
See Fig. 1.
Fig. 3. A: Golgi impregnated cholinergic
neuron (method of Gabbott and Somogyi, l984) located medial to the substriatal
gray. Neuron in boxed area is shown under higher magnification in B and C. D:
Electron micrograph showing gold deposits (arrows) in the perikaryon, in
addition to heavy immunostaining for ChAT. E: Low magnification view of
the identified neuron (# 1) in the electron microscope. Abbrev. CP = caudate putamen. Scale:
B=10µm; E=2µm.
Fig. 4. A: Camera lucida drawing (10x) from a
double-labeled section illustrating the distribution of PHA-L labeled fibers in
relation to cholinergic neurons following delivery of the tracer to the
orbitofrontal cortex. B: Schematic drawing (from section adjacent to the
one shown in A) illustrating the distribution pattern of PHA-L labeled
terminals in apposition to cholinergic neurons from a section that was analyzed
using high magnification light microscopy. Cholinergic neurons are represented
by dots. Zones of putative contacts between PHA-L-positive terminals and
cholinergic profiles are depicted as squares. Sections were screened using an
ocular reticle (80x80 µm) at 63x, and contact sites were marked on a camera
lucida drawing of the corresponding section using a proportional grid. Abbrev.
see Fig. 1.
Fig. 5. A: Degenerated terminal synapsing with a cholinergic dendrite in the ventral pallidum two days after lesion of the nucleus accumbens; b: for comparison a normal bouton. B,C: Degenerated terminals after a unilateral meso-diencephalic knife cut on cholinergic dendrites in basal forebrain. Arrows point to synaptic thickenings. Cholinergic profiles are indicated by asterisk. Bar scale: 1 µm.
Fig. 6. Proposed forebrain circuits involving the
cortex, basal ganglia and BFC neurons. Upper row: primate, lower row: rat. Data
from Alexander et al. (l986), Groenewegen et al., (1990), Haber et al. (l990).
Thick arrow marks the cholinergic link.
Abbrev. A = amyg-dala; ACg = anterior cingulate cortex; AId =
dorsal agranular insular cortex; c-MD = mediodorsal thalamic nucleus, central
part; d-GP = globus pallidus, dorsal part; dm-CAUD = caudate nucleus,
dorsomedial part; dm-VP = ventral
pallidum, dorsomedial part; E = entorhinal cortex; GPi = globus pallidus,
internal segment; H = hippocampus; l-ACb = nucleus accumbens, lateral
part; l-MD = mediodorsal thalamic
nucleus, lateral part; l-VP = ventral pal-lidum, lateral part; LOF = lateral
orbitofrontal cortex; m-ACb = nucleus accumbens, medial part; m-MD =
mediodorsal thalamic nucleus, medial part; m-VP = ventral pallidum, medial
part; MDmc = mediodorsal thalamic nucleus, magnocellular division; MOF = medial
orbitofrontal cortex; PRE = prelimbic cortex; vl-CAUD = caudate nucleus,
ventrolateral part; vm-CAUD = caudate nucleus, ventromedial part; vm-PUT; putamen,
ventromedial part; VA = ventral anterior thalamus.
Fig. 7. A: Low power electron micrograph of a
reconstructed cholinergic neuron from the substantia innominata (arrow in
B). Boxed area in A is enlarged at
inset in upper right corner, and show the synaptic contact of the PHA-L
varicosity, indicated by an arrow in C. Arrowheads in the inset denote sub-synaptic dense bodies. PHA-L was
injected into the lateral hypothalamus. Asterisks in A and C mark the same vessel
for orientation. Bar scale: A = 10 µm, inset = 1 µm. Reproduced from Záborszky
and Cullinan (1989) by permission of Elsevier Science Publishers.
Fig. 8. A,B: Degenerated terminals after
unilateral meso-diencephalic knife cut on cholinergic dendrites (asterisks) in
basal forebrain. Arrows point to postsynaptic thickenings. Bar scale: 1 µm.
Fig. 9. A: Hypothetical circuit involving the
auditory cortex, peripeduncular area (PPa), caudomedial (cm) striatum and
cholinergic and noncholinergic neurons in the caudal (c) globus pallidus in
rat. Heavy line on the right marks the putative cholinergic link. Other
connections of the PPa are indicated on the left, including those with auditory
subcortical relay stations (heavy lines). Data from Arnault and Roger (l987)
and LeDoux et al. (1990). Abbrev. ACe = central amygdaloid nucleus; AL =
lateral amygdaloid nucleus; CNf = cuneiform nucleus; DLL = dorsal nucleus of
the lateral lemniscus; IC = inferior colliculus; PAG = periaqueductal gray; PPT
= pedunculopontine tegmental nucleus; PRh = perirhinal cortex; VLL = ventral
nucleus of the lateral lemniscus; VMH = ventromedial hypothalamic nucleus; Zi =
zona incerta. B: Hypothetical circuit involving the insular cortex,
thalamic viscerosensory nuclei, parabrachial nucleus, nucleus of the solitary
tract, the central amygdaloid nucleus and cholinergic and noncholinergic
neurons in the SI in the rat. Hyphenated lines represent weaker
connections, heavy line the putative cholinergic link. Data from van
der Kooy et al. (l984), Shipley and Geinisman (l984), Cechetto and Saper
(l987), and Moga et al. (1990). Abbrev. PBl = lateral parabrachial
nucleus; PBm = medial parabrachial nucleus; VPLpc = parvicellular
ventroposterior lateral nucleus of the thalamus; VPMpc = parvicellular
ventroposterior medial nucleus of the thalamus.
Fig. 10. A: PHA-L injection site in the dorsal
raphe nucleus. The same section stained for serotonin (5-HT) (A') reveals that
many of the labeled neurons contain serotonin. B: A cholinergic neuron
from the dorsal aspect of the HDB (arrow in D) is approached by PHA-L fibers
with varicosities. C: Serotonin axon with varicosities in juxtaposition
to cholinergic neurons in the same area of intact animal. Note the similar
pattern of axonal arborization with respect to cholinergic neurons in B and C.
Scale; A, A', and D = 100µm; B and C = 10µm. Abbrev. ac = anterior
commissure; Aq = aqueductus cerebri; FStr = fundus striati; mlf = medial longitudinal
fascicle.
Fig. 11. A: PHA-L injection site in the
ventral medullary reticular formation. B: Enlarged view of the upper
part of the box of A showing several PHA-L labeled neurons. Arrow points to a
neuron which is double-labeled for dopamine-ß-hydroxylase (DBH). C: The
same section as in B stained for DBH. A number of noradrenergic cells of the A1
group are visible in the lower half of the picture. D: Combined
PHA-L/ChAT staining from the same case showing the distribution of PHA-L
labeled fibers in the forebrain in relation to cholinergic neurons. Bar Scale;
A = 500µm; C = 100µm. Star indicates the same vessel in B and C. Abbrev.
f = fornix; GP = globus pallidus.
Fig. 12. A: Distribution of
neurotensin-containing fibers/terminals and cholinergic cells at the level of
the posterior part of the crossing of the anterior commissure. B:
Schematic drawing from the same section showing that the ventral part of the
globus pallidus contains a number
of cholinergic projection neurons which
are embedded in a heavy neurotensin-containing network. Bar Scale: 500µm. Abbrev. ac = anterior
commissure; CP = caudate putamen; f = fornix; ic = internal capsule; MP =
medial preoptic nucleus; Tu = olfactory tubercle; 3V = third ventricle.
Fig. 13. A: Camera lucida drawing from a
frontal section at the level of the anterior commissure stained for
dopamine-ß-hydroxylase (DBH) fibers/terminals and cholinergic neurons using the
nickel enhanced DAB/DAB technique. Only the most proximal portions of dendrites
of cholinergic cells are drawn. B: Distribution of PHA-L labeled fibers
in relation to cholinergic neurons at the same level as A after PHA-L injection
in the locus coeruleus. Note that PHA-L labeled fibers/terminals from locus
coeruleus may represent a portion of those found in the DBH/ChAT material. Abbrev. ac = anterior commissure; HDB = horizontal
limb of the diagonal band.
Fig. 14. A: Topography of synaptic inputs to a
typical cholinergic neuron in the SI. Compiled
from our data as well as that of Bolam et al. (l986); Chang et al.
(l987) and Martinez-Murillo et al., (l988a, 1990). Putative inhibitory synapses
are labeled by solid, excitatory contacts by open symbols. B:
Somatostatin-containing bouton in synaptic connection with the dendrite. Double
labeling with nickel enhanced DAB/DAB. C: Bouton containing large
neurosecretory granula (small arrows) establishing synaptic contact with the
distal dendrite. Single immunostaining for ChAT. D: GABAergic bouton
labeled with ferritin (small arrow) establishes symmetrical contact with the
cholinergic cell body. From the material of Záborszky et al. (1986) by
permission of Willey and Liss. E: ChAT-positive bouton contacts proximal
ChAT-positive dendrite. Single immunostaining enhanced with cobalt. F:
DBH-positive bouton establishes asymmetric synapse with a cholinergic dendrite.
Double immunolabeling with NiDAB/ DAB. Arrowheads show subsynaptic dense
bodies. In all micrographs large arrows point to the postsynaptic side of the
synapse, asterisks label the immunostained (ChAT) profile. Bar scale for all
micrographs: 1 µm.
Fig. 15. A: PHA-L labeled terminal
varicosities in close apposition to a proximal dendrite of a cholinergic
neuron. The grid simulates the proportions of the ocular reticle used to screen
sections from high magnification (63x) light microscopic analysis. One division
of grid = 16 µm. B-F: Composite maps illustrating putative zones of contact
between afferent fibers and cholinergic neuronal elements following PHA-L
injections into the (B) locus coeruleus, (D) far-lateral
hypothalamus, (E) mid-lateral hypothalamus, (F) medial
hypothalamus. C: shows the distribution of putative contact sites from a
material stained for DBH/ChAT. Cholinergic neurons are represented by dots.
Zones of putative contacts between cholinergic elements and terminal
varicosities are depicted as solid squares (corresponding to 80x80 µm areas in
the section).